If you create a DOS bootable drive and use a non-US keyboard, Rufus will attempt to select a keyboard layout according to the locale of your system. In that case, FreeDOS, which is the default selection, is recommended over MS-DOS, as it supports more keyboard layouts. Rufus Free Download Latest Version for Windows PC. It is full offline setup installer of the software.
Creating an ISO image from a physical disc or from a set of files is very easy to do however, through the use of a CD burning application, such as the freely available CDBurnerXP or BurnAware.
It can be especially useful for cases where:
you need to create USB installation media from bootable ISOs (Windows, Linux, UEFI, etc.)
you need to work on a system that doesn't have an OS installed
you need to flash a BIOS or other firmware from DOS
you want to run a low-level utility